Document Type : Original Article
Authors
1
Departments of Livestock and Dairy Development, Animal Science Institute, Manager Production, Hi Tech Feed and Oil Mills, Quetta, Balochistan
2
Departments of Parasitology, Faculty of Veterinary Science, University of Agriculture ,Manager Production, Hi Tech Feed and Oil Mills, Quetta, Faisalabad
3
Faculty of Veterinary and Animal Sciences , Manager Production, Hi Tech Feed and Oil Mills, Azad Kashmir3, Pakistan
4
University of Agriculture , Manager Production, Hi Tech Feed and Oil Mills, Quetta Faisalabad ,Pakistan
5
Manager Production, Hi Tech Feed and Oil Mills, Quetta , Lahore ,Pakistan
6
Manager Production, Hi Tech Feed and Oil Mills, Quetta ,Sahiwal , Pakistan
7
Faculty of Veterinary and Animal Sciences, University of Poonch , Pathobiology (Parasitology)section, Manager Production, Hi Tech Feed and Oil Mills, Quetta ,Rawalakot, Azad Kashmir , Pakistan
Abstract
Background: Helminthic excretory secretory (E/S) antigens gained considerable interest due to their
role in stimulating host-parasite interactions and as potential candidate for vaccine development.
Objective: To quantify and characterize H. contortus E/S antigens aiming for its use as an effective control
measure.
Material and Methods: Adult worms were collected from the abomasa of slaughtered goats. Fresh and
highly motile adult worms were transferred to RPMI 1640 culture medium containing antibiotics. The
E/S antigen was procured through standard protocols and concentration of proteins was measured.
Characterization of ES antigen was subjected to SDS-PAGE and estimation of protein concentration.
Results: The triturate contained proteins with a concentration of 0.836 mg/ml. Three bands of proteins
appeared on the gel with molecular weights of 65, 48 and 30 kDa.
Conclusion: Further characterization of E/S antigens will be helpful in developing effective control
measures against the worms in general and haemonchosis specifically.
Keywords
)
View on SCiNiTO